Development and utilization of the functional co-dominant KASP marker for thermo-sensitive genic male sterility in rice Oryza sativa L.

Thermo-sensitive genic male sterility (TGMS) is an important genetic means of two-line hybrid rice breeding. Pollen fertility in TGMS lines regulated by a single point mutation genes. Based on nucleotide polymorphisms (SNPs) and insertion-deletion mutations, Kompetitive Allele-Specific PCR (KASP) markers were developed utilized molecular breeding via high-throughput detection, which saves time money. In this study, we converted the SNPs genes (including p/tms12-1and tms9-1) to functional co-dominant KASP bred resultant rice. We differentiated carrying p/tms12-1 or tms9-1 from other using assay. Pei’ai64S Hua201S (containing p/tms12-1) had homozygous GG genotype generating blue signal. HengnongS-1 CC red The assay for was identified as recessive Mendelian trait. Seed purity tested marker varieties Liangyoupeijiu Hualiangyou1206, consistent with findings dCAPS marker. Moreover, new generated pyramiding tms12-1 gene under same background. Therefore, detect study can be widely used It provides visually convenient toolkit breeders select individual target plants screening